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TcpB-mediated pilus retraction is required for CTX-Kmφ infection of V. cholerae. V. cholerae ΔtcpB was transformed with ptcpB encoding WT or Glu-5 substitutions. Cells were grown overnight in pilus-inducing conditions with 0.001 mm rhamnose to induce ectopic expression of TcpB. Cells from overnight culture were infected with CTX-Kmφ and plated on LB-Km. The number of cfu/ml for each infection is reported (n = 3); error bars represent the S.E.
