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. Author manuscript; available in PMC: 2019 Oct 28.
Published in final edited form as: J Vis Exp. 2019 Apr 9;(146):10.3791/59357. doi: 10.3791/59357

Figure 3: Wild type colonoids and shRNA transduced knockdown (KD) colonoids each form confluent monolayers.

Figure 3:

(A) Representative images of wild type (WT) human confluent colonoid monolayer differentiated for 5 days and (B) similarly grown monolayers derived from MUC2 KD colonoid cultures. Scale bar (A–B) = 50 μm (C) Representative immunoblot of colonoid cultures transduced with scrambled shRNA or MUC2 shRNA demonstrates that the changes in protein expression due to KD can be quantitated by immunoblot.