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. Author manuscript; available in PMC: 2019 Oct 28.
Published in final edited form as: J Vis Exp. 2019 Apr 9;(146):10.3791/59357. doi: 10.3791/59357

Figure 4: Enteroid/colonoid monolayers are suitable models to study luminal microbe-host interactions.

Figure 4:

(A) Representative maximum intensity projection and orthogonal optical section of a colonoid monolayer shows the thick apical MUC2-positive mucus layer which is not permeable to E. coli HS (black arrowheads) sitting at the apical mucus surface. The monolayer was infected for 6 hours with 106 cfu/mL HS. (B) Representative maximum intensity projection of human colonoid monolayer apically infected with EHEC (106 cfu/mL, 6 hours). Scale bar (A–B) = 50 μm. (C) MUC2 immunofluorescence intensity measurements show a significant decrease in MUC2 in EHEC-infected colonoid monolayers compared to uninfected controls. Error bars represent SEM.