TABLE 1.

Steady-State Intensities and Anisotropies for the Erythrosin B–[Ru(bpy)₃]²⁺ Mixture in Water at 20°C

[ErB] (μM)	[Ru(bpy)3]2+ (μM)	ITa	fErBb	r	$f_{\text{ErB}}^{\text{calc,}}$c
0	20	1275	0	0	0
0.5	20	1720	0.258	0.043	0.237
1	20	2250	0.433	0.075	0.414
2	20	3234	0.606	0.109	0.602
3	20	4081	0.687	0.122	0.674
4	20	5080	0.749	0.138	0.762
5	20	6035	0.789	0.144	0.796
6	20	7040	0.819	0.147	0.812
7	20	7705	0.834	0.149	0.823
8	20	8570	0.851	0.153	0.845

^aTotal fluorescence intensity, I_T = I_∥ + 2I_⊥.

^bFractional intensity of ErB. This value was calculated using f_ErB = (I − I_R)/I, where I_R is the intensity of the [Ru(bpy)₃]²⁺ in the absence of ErB.

^cFractional intensity of ErB calculated from the anisotropy data. This value was calculated using $f_{\text{ErB}}^{\text{calc}}=r/r_{\text{S}}$, where r_s is the anisotropy of ErB in the absence of [Ru(bpy)₃]²⁺