Kinetics of the binding of the AlexaFluor 647-labeled anti-rabbit antibodies to the rabbit IgG immobilized on a 50-nm silver mirror surface observed in buffer (blocking solution, ▲), human serum (■), and human whole blood (●) with the SPCE/KR configuration. Nonspecific kinetics (nonspecific binding of the AlexaFluor 647-labeled anti-rabbit antibodies to the immobilized goat IgG) is shown as corresponding open symbols unfilled triangles, squares, and circles. a.u., arbitrary units.