Table 5.

Associations Between Drusen Area and Volume and CFH and ARMS2/HTRA1

No. of Risk Alleles	Univariate Gene Analysis*					Bivariate Gene Analysis†
	n (Eyes)	Drusen Area Mean (mm2 ± SE)	P Value	Drusen Volume Mean (mm3 ± SE)	P Value	Drusen Area Mean (mm2 ± SE)	P Value	Drusen Volume Mean (mm3 ± SE)	P Value
CFH Risk Score‡
0-1	39	0.07 ± 0.16	Ref	0.0002 ± 0.008	Ref	0.25 ± 0.17	Ref	0.007 ± 0.008	Ref
2	103	0.49 ± 0.10	0.03	0.019 ± 0.005	0.04	0.57 ± 0.11	0.09	0.022 ± 0.005	0.11
3–4	97	0.66 ± 0.10	<0.001	0.029 ± 0.005	<0.001	0.74 ± 0.11	0.01	0.032 ± 0.005	0.006
P (trend)			0.004		0.002		0.01§		0.005§
ARMS2/HTRA1: rs10490924‖
0	105	0.19 ± 0.10	Ref	0.007 ± 0.005	Ref	0.15 ± 0.098	Ref	0.005 ± 0.0047	Ref
1	104	0.68 ± 0.10	<0.001	0.028 ± 0.005	0.002	0.60 ± 0.10	0.001	0.024 ± 0.005	0.004
2	30	0.90 ± 0.19	0.008	0.036 ± 0.009	0.004	0.80 ± 0.19	0.002	0.031 ± 0.0089	0.009
P (trend)			<0.001		<0.001		<0.001‖		0.001‖

^*Analyses based on continuous area and volume, adjusting for age, sex, education, body mass index, smoking, and AMD grade using the LSMEANS option of PROC MIXED of SAS. Each genetic factor was analyzed separately.

^†Analyses for CFH score are based on continuous area and volume, adjusting for age, sex, education, body mass index, smoking, AMD grade, and number of ARMS2/HTRA1 risk alleles using the LSMEANS option of PROC MIXED of SAS. Analysis for ARMS2/HTRA1 was performed similarly, adjusting for CFH score.

^‡No. of risk alleles for rs1410996 and rs1061170 combined.

^§The P trend assessing effect on drusen measurements per one category increase in CFH score was obtained after adjusting for number of ARMS2/HTRA1 risk alleles and other risk factors.

^‖The P trend assessing effect of increase in ARMS2/HTRA1 risk alleles was obtained after adjusting for categories of the CFH score and other risk factors.