miR-221 is an inducible miRNA by different strains of HCMV infection. (A) Gene Expression Omnibus (GEO) data analyzed by heatmap. (B) NPCs (Neural Precursor Cells) was infected with indicated doses of HCMV AD169 strain, and the expression level of miR-221 was evaluated by qRT-PCR. (C) NPCs was infected with HCMV AD169 strain for indicated hours, and the expression level of miR-221 was checked by qRT-PCR. (D) NPCs was infected with indicated doses of HCMV Towne strain, and the expression level of miR-221 was evaluated by qRT-PCR. (E) NPCs was infected with HCMV Towne strain for indicated hours, and the expression level of miR-221 was checked by qRT-PCR. These experiments were conducted three times, and the representative data were shown mean ± SD(* p < 0.05, ** p < 0.01 vs 0 h or 0 MOI).