Figure 4.

MiR-221 attenuates HCMV replication by promoting type I IFN signaling.(A-D) NPCs cells were transfected with siRNAs targeting IFNAR1 and IFNAR2 or si-scramble for 24h, followed by infected with HCMV at MOI of 1 for 24 h. Cells were harvested for mRNA and real-time PCR was conducted to determine the expression of IFNA receptor (A), ISGs expression like ISG15(B), MxA(C), OAS1(D). (E-F) NPCs cells were transfected with miR-221 mimics alone or co-transfected miR-221 mimics with si-IFNAR1 or si-IFNAR2 for 24h, followed by infected with HCMV at MOI of 1 for 24hr. (E) HCMV DNA copy number per cell was determined by qRT-PCR and standardized to cellular DNA (GAPDH) copy number. (F) Titers of infectious virus in the culture supernatants were determined by plaque assay. All these experiments have been repeated 3 times, and the representative data were shown. (* p < 0.05, ** p < 0.01 vs si-scramble group or HCMV Infection group).