Figure 1.

The cross-binding and cross-neutralizing abilities of MAb NA11F12. (A) Immunofluorescence assay of the MAb NA11F12 against the CA16-infected RD cells. Uninfected RD cells were used as the negative controls. The secondary antibody was FITC-conjugated anti-mouse (green). The nuclei were stained with DAPI (blue). (B) NA11F12 (6.25 μg) bound specifically with CA16 strains (5 × 10⁴CCID⁵⁰) covering A, B1, B2, C subgenotypes but not EV71 by ELISA. (C) The positive bands of MAb NA11F12 reacted with A, B1, B2, C subgenotypes CA16 strains were found at about 32 kDa. (D) Neutralizing titers of MAb NA11F12 against CA16 covering A, B1, B2, C subgenotypes by neutralization assay. The Bonferroni’s Multiple Comparison Test was used to compare the difference of the neutralizing titer. **The neutralizing titer against EV71 was significant difference with other groups, p < 0.05. *The titer against CA16 C subgenotype was significant difference with other CA16 subgenotypes, p < 0.05.