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(a) UV chromatograms of NIST mAb subunits obtained using 0.1% FA, DFA, or TFA-modified mobile phases, and (b) total ion current chromatograms (TICs) of NIST mAb subunits obtained using 0.1% FA, DFA, or TFA-modified mobile phases. Separations were performed with a high coverage phenyl-bonded superficially porous silica 450 Å, 2.7 μm, 2.1 × 50 mm column using a flow rate of 0.2 mL/min, column temperature of 80°C, and 0.25 μg mass loads.
