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. 2019 Sep 10;11(8):1358–1366. doi: 10.1080/19420862.2019.1658492

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© 2019 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 4. — MS peak intensities of oxidized and extended C-terminal Fc/2 subunits from a cysteine-linked auristatin conjugated as determined by the (a) method consisting of a C4-bonded organosilica 300 Å fully porous stationary phase, 0.6 mL/min flow rate, 80°C temperature, 0.1% TFA-modified mobile phases, and 90:10 acetonitrile/IPA eluent versus (b) the new method consisting of a phenyl bonded 2.7 µm superficially porous 450 Å stationary phase, 0.6 mL/min flow rate, 70°C temperature, and 0.15% DFA-modified mobile phases.