Table 1.
Variation in method output (RSD, %) as a function of robustness testing for a technique comprised of evaluating changes in stationary phase batches, DFA batches, LC systems, columns, temperature, mass load, flow rate, DFA concentration, and lifetime for the separation observed in Figure 3(c).
| Variation in Method Output (RSD, %) | ||||||
|---|---|---|---|---|---|---|
| Peak Capacity | Percent Peak Area of Light Chain(0) | Percent Peak Area of Fd′(3) | Retention Time of Light Chain(0) | Retention Time of Fd′(3) | DAR | |
| Stationary Phase Batches (3) | 3.7 | 1.4 | 3.1 | 1.9 | 1.8 | 0.2 |
| DFA Batches (3) | 0.6 | 0.8 | 2.6 | 0.7 | 0.4 | 0.4 |
| LC Systems (3 Systems, 9 Columns) | 1.7 | 3.1 | 4.3 | 0.5 | 0.4 | 0.5 |
| Temperature (±5%) | 3.7 | 1 | 1.4 | 1.1 | 0.3 | 1.1 |
| Mass Load (±5%) | 0.9 | 0.4 | 1.9 | 0.2 | 0.2 | 0.2 |
| Flow Rate (±5%) | 0.7 | 0.2 | 0.8 | 1.1 | 0.8 | 0.1 |
| Percent DFA (±5%) | 4.7 | 2.7 | 4.6 | 2.0 | 0.9 | 0.9 |
| Lifetime Study (1) | 1.6 | 1.8 | 4.0 | 0.5 | 0.3 | 0.0 |
| Lifetime Study (2) | 0.3 | 0.2 | 1.2 | 0.7 | 0.8 | 0.4 |