Table 4.
PK parameters of heterodimeric IgGs following a single IV bolus injection in female Sprague-Dawley rats.
| PK parameter | Fab-scFv dG-Fc | Fab-scFv dA-Fc | Fab dA-scFv dG-Fc | BEAT* Fab dA-scFv-Fc |
|---|---|---|---|---|
| Cmax (µg/ml) | 383 ± 42 | 237 ± 14 | 308 ± 18 | 230 ± 28 |
| AUC0-inf (hr*µg/ml) | 39507 ± 1755 | 26099 ± 3013 | 29314 ± 2179 | 26371 ± 116 |
| CL (ml/day/kg) | 6.1 ± 0.3 | 9.3 ± 1 | 8.2 ± 0.6 | 9.1 ± 0.05 |
| Vss (ml/kg) | 58 ± 5 | 108 ± 8 | 84 ± 7 | 98 ± 3 |
| t1/2 (day) | 8.1 ± 0.6 | 9.5 ± 0.4 | 8.2 ± 0.4 | 7.9 ± 0.2 |
(*) denotes the presence of the L234A and L235A substitutions in both BEAT Fc chains (Eu numbering). (AUC0-inf) area under the serum concentration-time curve from time zero to infinity. (CL) clearance. (Cmax) maximum serum concentration. (t½) elimination half-life. (Vss) apparent volume of distribution at steady state. Standard deviation for each parameter is indicated.