Table 2. Amplification protocols for PCR by primers set.
Primer | Denaturing | Annealing | Elongation | Cycles |
---|---|---|---|---|
MY09 | 94 °C/50 s | 55 °C/50 s | 72 °C/55 s | 38 |
MY11 | ||||
PVf | 94 °C/1 min | 50 °C/1 min | 72 °C/1 min | 45 |
FAP64 | ||||
CP4 | 94 °C/30 s | 42 °C/30 s | 72 °C/30 s | 40 |
CP5 | ||||
GH20 | 94 °C/50 s | 55 °C/50 s | 72 °C/55 s | 38 |
PC04 | ||||
dogGAPDHf | 94 °C/30 s | 50 °C/30 s | 72 °C/30 s | 45 |
dogGAPDHr |