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. 2019 Oct 25;7:e7962. doi: 10.7717/peerj.7962

Table 2. Amplification protocols for PCR by primers set.

Amplification conditions used in this study are indicated.

Primer Denaturing Annealing Elongation Cycles
MY09 94 °C/50 s 55 °C/50 s 72 °C/55 s 38
MY11
PVf 94 °C/1 min 50 °C/1 min 72 °C/1 min 45
FAP64
CP4 94 °C/30 s 42 °C/30 s 72 °C/30 s 40
CP5
GH20 94 °C/50 s 55 °C/50 s 72 °C/55 s 38
PC04
dogGAPDHf 94 °C/30 s 50 °C/30 s 72 °C/30 s 45
dogGAPDHr