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Chromatograms from purifications of mAbs on the ZCaTetraCys column using different pH. (a) Elution of human IgG1 (red), IgG2 (yellow) and IgG4 (green) using a gradient of 0–200 mM EDTA (gray) at pH 5.5. IgG1 required a higher EDTA concentration than IgG2 and IgG4 to be eluted. (b) Elution of IgG1 (red), IgG2 (yellow) and IgG4 (green) using a gradient of 0–200 mM EDTA (gray) at pH 7. All bound IgG2 and IgG4 could be eluted at a neutral pH whereas most IgG1 was retained on the column.
