Gm26809 overexpression enhanced the B16F0-Exo-mediated transformation of fibroblasts into CAFs.
(a) qRT-PCR analysis of Gm26809 in NC-Exo and B16F0-Exo. (b) qRT-PCR analysis of Gm26809 in LV-NC-Exo and LV-Gm26809-Exo. qRT-PCR analysis of Gm26809 expression (c), western blot analysis of α-SMA and FAP protein levels (d), and cell migration determined by Transwell migration assay (E) in NIH/3T3 cells which were stimulated with B16F0-Exo, LV-NC-Exo and LV-Gm26809-Exo (20 μg/ml) for 24 h. (F) Confocal microscope images confirmed the uptake of exosomes in NIH/3T3 cells. FITC: FITC-tagged Gm26809 (green), PKH26: PKH26-labeled exosomes (red), DAPI: cell nuclei (blue). Scale bar, 10 μm. Values are represented as means ± SD (n = 3). **P< 0.01, vs. LV-NC-Exo.