Figure 5.

Fibroblasts treated with melanoma-derived exosomes facilitate melanomagenesis, which can be rescued by Gm26809 knockdown.

Mouse melanoma cells Cloundman S91 were cultured by the culture media of NIH/3T3 cells, which were stimulated with or without B16F0-Exo, LV-Scramble-Exo, and LV-shGm26809-Exo (20 μg/ml) for 24 h. (a) MTT assay was performed to evaluate Cloundman S91 cell proliferation. (b) Transwell migration assay was conducted to evaluate the migration ability of Cloundman S91 cells. Scale bar, 50 μm. Values are represented as means ± SD (n = 3). *P< 0.05, **P< 0.01, vs. Control. ^#P< 0.05, ^##P< 0.01, vs. LV-Scramble-Exo.