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Optimization of the parameters used for the in vitro neutralization assays. (a) Selection of the sensitive cell line. For each cell line, the same number of cells was infected with equal amounts of pRVFV, and the RLU values were detected concurrently. (b) Optimization of the viral inocula. The ID50 value of the serum from an immunized guinea pig was determined using different pRVFV doses.
