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. 2019 Sep 2;11(8):1479–1491. doi: 10.1080/19420862.2019.1658493

Figure 3.

Figure 3.

Fluorescence intensity of IgG4-N1 VIA and VIN samples with various hold times. All samples except the buffer control had the same protein concentration. The excitation wavelength of Sypro Orange was 490 nm, with emission scanned from 500 nm to 700 nm. (a) VIA samples held at pH 3.3 for various times (10 min-3 h) before adding Sypro Orange for measurements by fluorescence spectroscopy. (b) VIN samples (from VIA at pH 3.3 held for 1 h) held at pH 5.5 for various times (0 min-48 h) before adding Sypro Orange.