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. 2019 Sep 2;11(8):1479–1491. doi: 10.1080/19420862.2019.1658493

Figure 4.

Figure 4.

Inline fluorescence SEC data for VIA and VIN samples with various hold times. Sypro Orange signal was collected for excitation at 490 nm and emission at 574 nm. (a) Inline fluorescence overlay of VIA samples at pH 3.3 with various hold times (10 min-3 h). Peak maxima correspond to 8.25 min retention time, shown as vertical dotted line. (b) UV 280 nm signal overlay of the same samples in (a). (c) Fluorescence signal overlay of VIN sample at pH 5.5 with various hold times (10 min-24 h), using a VIA pool held at pH 3.3 for 1 h. (d) UV 280 nm signal overlay of the same samples in (c).