Figure 5.

Addition of novel anti-IL-26 mAbs inhibits proliferation and tube formation of IL-26-stimulated HUVEC.

HUVEC were stimulated with recombinant human IL-26 (10 ng/ml) for 48 hr (a) or 9 hr (b). Prior to the onset of stimulation, the indicated Ab or isotype control Ab (isotype ctrl) was added to the culture wells to give a final concentration of 20 μg/ml each. (a) Proliferation was assessed by cell confluence. (b) Tube formation was assessed by cell sprouts formation. Representative images are shown in the right panels. Scale bar, 300 μm. Quantification of tube length is demonstrated in the graph in the left panel. (a, b) The dashed line is the standard value of unstimulated cells (vehicle). Representative data of three independent experiments are shown as mean ± S.D. of triplicate samples, comparing values in each Ab to those in isotype control (* p < 0.01), and similar results were obtained in each experiment.