Figure 6.

Addition of novel anti-IL-26 mAbs hardly affects the antimicrobial activity of IL-26.

(a) Immobilized recombinant human IL-10 (rhIL-10), IL-22 (rhIL-22), IL-26 (rhIL-26) or vehicle (PBS) alone was incubated with the indicated concentrations of purified LPS (left panel) or LTA (right panel). The absorbance at 450 nm/570 nm was measured. Representative data of three independent experiments are shown as mean ± S.D. of triplicate samples, and similar results were obtained in each experiment. (b, c) Growth kinetics of Escherichia coli (b) and Staphylococcus aureus (c) cultured with 10 μM (left panels) or 5 μM (right panels) rhIL-26, LL-37 or human β-defensin 3 (hBD3). (d) Escherichia coli was cultured with 10 μM rhIL-26 for 6 hr. Prior to the onset of culture, the indicated Ab or isotype control Ab (isotype ctrl) was added to the culture wells to give a final concentration of 150 μg/ml each. (b-d) Serial dilutions of bacterial cultures were plated onto agar plates, and the number of colonies was counted. Representative data of three independent experiments are shown as mean ± S.D. of triplicate samples, and similar results were obtained in each experiment.