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. 2019 Jun 27;65(4):312–325. doi: 10.1080/19396368.2019.1625085

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© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Normalized qPCR results for verification of DE analysis. Following 2^−ΔΔCt normalization and calculation relative to GAPDH expression (stable among our samples), the more highly expressed of the pairs in each case were set to 1.0 (arbitrary units). The chart shows that among the incompetent blastocysts, HSD17B1, CYP11A1, and DHCR7 were significantly down-regulated, while BAK1 and KHDC1P1 were significantly up-regulated. Standard deviation error bars are also displayed in the graph. These results concord with the edgeR differential expression analysis obtained from the RNA-seq data.