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. Author manuscript; available in PMC: 2019 Oct 28.
Published in final edited form as: Mod Pathol. 2014 Sep 5;28(2):187–200. doi: 10.1038/modpathol.2014.109

TABLE 1:

Conditions for Immunohistochemical Stains.

Marker Clone Source Dilution Antigen retrieval
S100 S100 Rabbit polyclonal Ventana 1:2 CC1-120 min
SOX10 SOX10 Rabbit polyclonal Cell Marque 1:50 CC1-60 min
Neurofibromin NFC Mouse monoclonal DKFZ 1:4 CC2-56 min
SOX2 AB5603 Rabbit polyclonal EMD Millipore 1:200 CC1-32 min
p75NTR NGFR5 Mouse monoclonal Abcam 1:100 CC1-32 min
p16 JC8 Mouse monoclonal Santa Cruz 1:50 CC1-32 min
p53 DO-7 Mouse monoclonal Dako 1:50 CC1-32 min
EGFR 3C6 Mouse monoclonal Ventana 1:2 CC1- 32 min
MIB1 30-9 Rabbit monoclonal Ventana 1:1 CC1-32 min

CC1: Cell conditioner 1 (pH 9), Ventana (Ventana Medical Systems, Tucson, AZ, USA); CC2: Cell conditioner 2 (pH 6), Ventana (Ventana Medical Systems); min: Minutes.