Figure 1:

Knockdown of PARG with shPARG decreases PDAC tumor growth in vivo and in vitro. (A) Athymic nude mice bearing MIA.shPARG (left) xenografts were randomized into -DOX and +DOX arms and relative fold change in tumor volume was plotted. *P<0.05 using two-sample t-tests. And representative images (right) of tumor extracted after completion of in vivo study from MIA.shPARG mice (n=5 per group). (B) Relative mRNA expression of PARG and PARP in harvested tumors from MIA.shPARG (−/+ DOX) in vivo. Mean±SEM, n=3,*P =0.01 to 0.05.(C) In vitro fold change in growth of Lentiviral.shPARG PANC-1 cells compared to shScramble. (D) Lentiviral shPARG knockdown enhances sensitivity of cells to oxaliplatin and (E) decreases sensitivity to olaparib in PANC-1 (top) and MIA PaCa-2 (bottom) cells in vitro. Representative graphs from n=3 are shown, *P <0.05 using two-sample t-tests.