PARG inhibition is synthetic lethal with HR deficiency in PDAC cells. (A) Pico Green assays were performed after 5 days of treatment with PDDX-001, PDDX-002 and PDDX-004 to analyze relative cell survival in PDAC cells (MIA Paca-2, PANC-1). (B, C and D) Relative cell survival of MIA PaCa-2 and Hs 766T treated with PDDX-001 over 3, 5 and 7 days; KPC BRCA WT and KPC BRCA2 null cells as well as pancreatic cancer patient derived xenograft cell lines (SPC_126, SPC_122, SPC_144). (E) Synthetic lethality with PDDX-01 and siRNA DDR genes in MIA PaCa-2 and PANC-1 cells. Representative graphs from n=2 for (A) and n=3 for (B, C, D and E) are shown.