Fig 1. Curation of exon 3 of PIN9 (Zm00001d043179).

Exons of incorrect length were the most common error detected by both triage methods. The Apollo editing window shows a “User-created annotation” at top followed by the longer, incorrect B73 RefGen_V4 model (“MAKER_updated”). The shortened exon was supported by aligned evidence: protein sequences from sorghum and rice, assembled long Iso-Seq reads combined from six tissues, and RNA-seq from roots, among other tissues.