Fig 4. Curation of two mini-exons in INVCW4 (Zm00001d001941).

The Apollo editing window shows a “User-created annotation” at top, followed by incorrect B73 RefGen_V4 model (“MAKER_updated”) and “v3 model mapped to v4.” A conserved 9-nucleotide exon (red circle) and a novel 19-nucleotide exon (blue circle) were supported by protein sequences from sorghum and rice, assembled EST transcripts from ultra-deep sequencing, long Iso-Seq reads combined from six tissues, and RNA-seq from root and other tissues.