Fig 6. E-cadherin-Pten bigenic knockout results in increased invasion, inflammation & goblet cell metaplasia in the prostate.

A. Gene construct of the targeted Pten floxed allele in addition to the previously described Cdh1 targeted allele undergoing recombination in the presence of PB-Cre4. B. Table summarizing the pathological phenotypes observed in Cdh1^L/L:Pten^L/L:PB-Cre4 vs Pten^L/L:PB-Cre4 control mice by age. C1-C4’ Representative images of key pathological features observed in 5–10 month old Cdh1^L/L:Pten^L/L:PB-Cre4 mice. Pathological features include invasive adenocarcinoma with goblet cell metaplasia indicated by arrows (C1’). Red arrows indicate areas with goblet cell metaplasia (C1’and C2’). D1-D2’ Representative images of typical PIN features observed in 5–10 month old Pten^L/L:PB-Cre4 control mice. E1-F6. Representative images of H&E and IHC staining using the indicated antibodies from adjacent prostate tissue sections from Cdh1^L/L:Pten^L/L:PB-Cre4 and Pten^L/L:PB-Cre4 mice. Red arrows indicate E-cadherin negative cells (E2). Scale bars, located in the bottom left corner of images, are sized as indicated.