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. 2019 Oct 28;14(10):e0224113. doi: 10.1371/journal.pone.0224113

Fig 4. CRISPRa affects IL6 expression in HEK239T.

Fig 4

A, regulation of RP11 transcription by repressive (KRAB) or activating (VP160) Cas9 derivatives. Cells were transfected with an activator or a repressor, in the presence of SG-286 or trcrRNA alone. Set used was independent from Fig 5. Data from 3 independent experiments (except for [IL6/ RP11] + KRAB, 5 experiments). B, CRISPRa activation increases secreted IL6 levels. IL6 protein concentration was measured in the media of HEK293T cells transfected for 48 h with CRISPRa and either SG-286 or trcrRNA alone.