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Time course of SG-286 activation in HEK293T. Cells were transfected with dCas9-VPR and SG-286 or trcrRNA for the indicated times. Indicated RNAs were quantified by qRT-, normalized to PPIA values and then divided by the trcrRNA/dCas9-VPR. Data represent the average of 3 experiments (± S.D.).
