Fig 8. Kinase inhibitors interfere with CRISPRa activation of IL6.

HEK293T cells were transfected for 24 h with dCas9-VPR together with the indicated sgRNA constructs, in the presence of kinase inhibitors. Cells were then harvested and IL6, SRP14 and PPIA levels were quantified. Values for IL6 (relative to PPIA) are shown; SRP14 quantifications are shown in S4 Fig. Values are normalized to the corresponding vehicle values to emphasize the impact of the drugs on each CRISPRa transfection and facilitate inter-construct comparisons. Data represent the average of 3 experiments (± S.D.).