Table 1. Over Representation analysis of transcription factor targets.

Motif	Size	Expect	Ratio	P Value	FDR
TGGNNNNNNKCCAR_UNKNOWN	409	2.576	3.8821	0.000232	0.082281
IK3_01	213	1.3415	5.218	0.000377	0.082281
GTGGGTGK_UNKNOWN	285	1.795	4.4569	0.000412	0.082281
NKX61_01	226	1.4234	4.9179	0.000538	0.082281

Over Representation Analysis of transcription factor targets via WebGestalt. The analysis looks within a gene list for a particular pattern that is enriched in a subset of gene. In the current analysis, promoter sequences of the nominally affected genes and the entire gene list are first screened for the presence of putative as well as validated transcription factor binding sites. Binding sites that are enriched in the normally affected list (i.e. more present than expected by chance) may indicate shared underlying effectors. RNAs isolated from HEK293T cells transfected with dCas9-VP160 alongside either trcrRNA or SG^-286 were subjected to transcription array analyses. Transcripts showing more than 2-fold and nominally significant (p<0.05) changes were mapped to 135 unique Entrezgene IDs; more speculative genes (Pseudogenes, lncRNA and Aceview transcripts) were excluded from the analysis. Of the 135 IDs, 76 were annotated to the functional categories and were present in the reference list. The reference list was mapped to 21491 Entrezgene IDs of which 12067 were annotated to functional categories. Multiple comparison correction was performed by False Discovery Rate according to Benjamini Hochberg and only the FDR values lower than 0.1 are shown; FDR significance threshold is 5% (0.05). Size, number of genes in the reference list; Expect, expected representation; Ratio, ratio of Obtained/Expected; P value, nominal p value; FDR, False Discovery Rate (q value).