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. 2019 Oct 28;14(10):e0224297. doi: 10.1371/journal.pone.0224297

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© 2019 Fry, Carter

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — Representative ion chromatograms (m/z 44) and ion ratio plots (45/44) for the PSIA analysis of a keratin hydrolysate (USGS42 Tibetan human hair) showing; (A) the short HPLC method and (B) the long HPLC method. Peaks that were integrated to calculate the final results are labelled. In both chromatograms the working gas (WG) was set to an intensity of 4000 mV and the 45/44 ratio swing for the Arg peak was typically 1.16 to 1.24. The 45/44 ratio swing for the Gly peak varied between the short run (typically 0.9 to 1.4) and the long run (typically 1.10 to 1.45) due to different solvent compositions and different interaction with the ionic stationary phase.