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. 2019 Oct 28;14(10):e0224297. doi: 10.1371/journal.pone.0224297

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© 2019 Fry, Carter

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 4 — Performance considerations for the PSIA method; (A) the decarboyxlation CO₂ yields for amino acids (mean ± sd n = 4) obtained with the pH 1.5 ninhydrin reagent, (B) the difference (Δ¹³C_CARBOXYL) between amino acids decarboxyled with pH 5.8 and pH 1.5 ninhydrin reagents and, (C) a comparison of amino acid δ ¹³C_CARBOXYL values determined by flow injection analysis (no HPLC) of individual RMs and the same materials analysed by HPLC short (squares) and long (circles) runs.