Table 1. Solvent compositions for short and long HPLC runs.
| short run | long run | ||||||
|---|---|---|---|---|---|---|---|
| time (min) | A (%) | B (%) | C (%) | time (min) | A (%) | B (%) | C (%) |
| -50 pre-run (RMs) | 0 | 0 | 100 | ||||
| -36 pre-run (RMs) | 100 | 0 | 0 | ||||
| -14 pre-run (RMs) | 99.7 | 0.3 | 0 | ||||
| 0 | 99.7 | 0.3 | 0 | 0 | 100 | 0 | 0 |
| 35 | 100 | 0 | 0 | ||||
| 40 | 99.7 | 0.3 | 0 | ||||
| 80 | 0 | 100 | 0 | ||||
| 106 | 0 | 100 | 0 | ||||
| 153 | 97.2 | 2.8 | 0 | ||||
| 154 | 32.5 | 67.5 | 0 | ||||
| 200 | 32.5 | 67.5 | 0 | ||||
| 201 | 0 | 100 | 0 | ||||
| 210 | 0 | 100 | 0 | ||||
Elution profiles listed here were adjusted as the HPLC columns aged and retention properties changed. Reference materials (Glu and Asp) were introduced during the equilibration period (-50 to -14 min) prior to sample injection (0 min).