Fig 5. All trackable mutations can be used as MRD markers in post-transplant setting.

(A) Shown are 10 MRD positive patients where additional fully-engrafted samples (via STR PCR/histology) were available in the > 100 day time-period prior to relapse. The VAF of the trackable pathogenic variants are followed over a time period of 0 to 220 days prior to relapse. In all 10 cases, we were unable to detect trackable variants at >100 day time points. (B) VAF of all trackable variants from all MRD-positive RG samples starting at relapse to 220 days prior. All pathogenic variants detected at relapse diminished over the course of time, with no variants detected at >80 days. In both Fig 5A and 5B, VAF cutoffs of 0.1% and 0.001% were used for point mutants (green dotted line) and indels (red dotted line) respectively and data points where %VAF was below the detection limit is plotted at detection limit but denoted with open circles while closed circles represent the measured %VAF.