Figure 2.

Lentiviral transduction of enzymatically isolated ovarian follicles and the dynamic activities of transcription factors. (a) Construct of the luciferase reporter. TRE: transcription factor respond element; TA: tyrosine kinase promoter; (1) fusion of the lentivirus to granulosa cells; (2) reverse transcription; (3) integration to host cell genome; (4) specific transcription factors translocating to the nucleus; (5) and (6) start of gene transcription; (7) translation of the luciferase protein; (8) detection of luminescence. (b) Illustration of lentiviral transduction of enzymatically isolated ovarian follicles; (c) representative bioluminescence images of transduced follicles to confirm luciferase expression. Scale bar = 100 µm; (d–g) dynamic activities of selected TFs (NF-κB = d, HIF1 = e, Gli = f, and p53 = g) during folliculogenesis.