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HDAC4 localization is influenced by substrate stiffness. (a) Immunofluorescence images of HDAC4 with and without ERK inhibition in fibroblasts cultured on soft (0.5 kPa) and stiff (100 kPa) polyacrylamide hydrogels. (b) Quantitation of fluorescence intensity. N = 3. Error bars represent ± SEM. **p value <0.01; scale bar = 90 µm.
