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. 2019 May 7;12(3):215–226. doi: 10.1007/s12195-019-00570-7

Figure 2.

Figure 2

(a) Brightfield image (objective: × 60) of nodules after 3 days of culture on Matrigel method. (b) Brightfield image (objective: × 10) of nodule on day 4 cultured using the low attachment method. (c) Depth in the z axis (µm) vs. natural log of normalized intensity for Matrigel (N = 5) and low attachment (N = 5) nodules. A comparison of liner fits shows significant differences (p ≤ 0.0001) between methods in signal decay.