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(a) Example of nodule attached to collagen coated measured by AFM. Three force maps per nodule spanning an area of 2500 µm2 each were measured. (b) Increased osmolality caused an increased Young’s modulus measured by AFM. (c) Example of nodule being contacted by Microsquisher parallel compression plate. (d) Increased osmolality exhibited an exponential relationship with Young’s modulus measured by the Microsquisher.
