Table 2.

Summary of techniques and culture method, duration, conditions, and number of samples for mechanical analysis.

Methods summary
Technique	Culture method	Culture duration (days)	Conditions (sucrose concentration mM)	Samples per condition
Brillouin	Matrigel	3	0, 500, 1000	9 nodules
Brillouin	Low attachment	4	0, 62.5, 125	6 nodules
AFM	Low attachment	4	0, 500, 1000	3 nodules (3 regions of interest per nodule)
Microsquisher	Low attachment	4	0, 250, 500, 750, 1000	8, 7,7, 7, 6 nodules respectively