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. 2019 May 21;12(5):357–373. doi: 10.1007/s12195-019-00576-1

Figure 3.

Figure 3

DiLNs Synthesis and Characterization: (a) PI103 and Selumetinib loaded liposomes were highly unstable with significant precipitation observed within 10 min of synthesis. (b) Structure of the P13K-inhibiting amphiphile. (c) Structure of the MAPK-inhibiting amphiphile. (d) Schematic representation shows assembly of DiLNs from phosphatidylcholine (PC), P13K inhibitor amphiphile, MAPK inhibitor amphiphile and DSPE-PEG-Amine. (e) High resolution cryo-transmission electron microscopy image (scale bar, 100 nm) of the DiLNs. Insert shows the hydrodynamic size distribution of DiLNs obtained through dynamic light scattering (DLS). (f) Graph shows the physiochemical stability of the DiLNs as a function of the change in size distribution and zeta potential, measured by DLS over a 30-day period. (g) DiLNs maintained the stability for over 30 days with no precipitation observed. (h) Graph shows the stability of the DiLNs when incubated with human serum as a function of the change in size and zeta potential, measured by DLS. (i) Release kinetics profiles of PI3K and MAPK inhibiting amphiphiles at acidic pH (5.5) and physiological pH (7.4). (j) Release kinetics profiles of PI3K and MAPK inhibiting amphiphiles in TOV21G cell lysate.