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. 2019 Sep 24;12(5):511–528. doi: 10.1007/s12195-019-00598-9

Figure 1.

Figure 1

(a) In the dark state, the Jα helix in LINuS is folded and interacts with the AsLOV2 core domain. This sequesters the nuclear localization signal (NLS) and prevents it from interacting with importins. Upon blue light exposure, the Jα helix unfolds rendering the NLS accessible. (b) Schematic of the dCas9-LINuS and dCas9-mCherry-LINuS used in this study. pTDH3 is a constitutively strong promoter. (c) Blue light induces unfolding of the Jα helix, allowing endogenous importins to interact with the nuclear localization signal allowing dCas9-mCherry-LINuS to be imported into the nucleus where it sterically inhibits transcription by binding to promoters or coding regions as guided by the appropriate sgRNA.