Figure 11.

Repressors in Fig. 10 were assembled into multigene cassettes containing a guide RNA for the ERG11 gene. Repression of ERG11 confers sensitivity to the antifungal drug fluconazole. Dilutions (1:1, 1:10, 1:100, 1:1000 left to right) of cells were spotted onto plates containing 32 mg/mL fluconazole and grown in ambient light. The strain without a repressor shows no additional fluconazole sensitivity relative to wild-type cells. Sensitivity in strains carrying the dCas9 repressors agrees qualitatively with the repression of Tef1-GFP seen in Fig. 10a.