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. 2019 Sep 24;12(5):511–528. doi: 10.1007/s12195-019-00598-9

Figure 5.

Figure 5

Yeast strains carrying dCas9-Mxi1-mCherry-LINuS with or without the sgTEF1 guide RNA were compared for repression in light and dark. (a) Co-transformation of dCas9-Mxi1-mCherry-LINuS with sgTEF1 leads to a 5-fold repression in median Tef1-GFP expression with or without light relative to the no guide control (median fluorescence, n = 3, p < 0.01, Welch’s t-test). (b) Violin plots demonstrate the increase in expression range in the dCas9-Mxi1-mCherry-LINuS + sgTEF1 strains with or without light relative to the control. Violin plots represent median, interquartile range, and 95% confidence interval on a representative population of cells (biological replicates).