Figure 9.

We generated dCas9, LINuS, and Mxi1 as either coding sequence or terminator parts, with and without stop codons and constitutive NLSs to allow for versatile repressors to be constructed using the toolkit. Part plasmids contain unique upstream and downstream BsaI-generated overhangs to assemble into the appropriate position in “Cassette” plasmids. Cassette plasmids are fully functional transcriptional units that are further assembled into multigene plasmids using BsmBI assembly and appropriate Assembly Connectors. This figure utilizes the color scheme and organization from Lee et al.33 and An-Adirrekun et al.1 to illustrate how the new optogenetic components integrate with the existing yeast toolkit. (P, Promoter; T, Terminator; S, Scar)