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. 2017 Aug 28;10(5):501–513. doi: 10.1007/s12195-017-0506-7

Figure 2.

Figure 2

Tethered Jagged-1 synergizes with matrix stiffness to promote Notch NICD nuclear translocation. (a) Representative immunocytochemical images of primary myoblasts stained for NICD (green) and Draq5 (red). Arrows point to examples of nuclear (left) and cytoplasmic (right) NICD localization. Scale bar, 10 µm; (b) Bar graph showing the proportion of cells exhibiting nuclear NICD localization when exposed to soluble (checkered) or tethered (solid) IgG control (white) or Jagged-1 (Jag1; gray) Notch ligand in the context of 4, 12, or 42 kPa PEG hydrogels. Error bars indicate mean ± SEM. n = 3 and P < 0.05 by two-way ANOVA where indicated.