Figure 3.

Tethered Jagged-1 in differentiation conditions does not modify myoblast proliferation. (a) Representative immunocytochemical images of murine myoblasts stained for Hoechst (blue; left) and EdU (green; middle) that are then merged to show a composite of the two images (right). Scale bar, 50 µm; (b) Bar graph quantifying the proportion of myoblasts that incorporate EdU in the last 12 h of a 48-h period in differentiation media when exposed to tethered IgG1 (white) or tethered Jagged-1 (Jag1; gray) in the context of 4, 12, or 42 kPa hydrogels. n = 3 and P = not significant by two-way ANOVA; (c) Bar graph indicating the total number of primary myoblasts following 72 h of incubation in differentiation media. n = 3 and P = not significant by two-way ANOVA.