Figure 4.

Tethered Jagged-1 synergizes with substrate stiffness to influence primary myoblast fate in culture. (a) Representative immunocytochemical images of primary myoblasts stained for Hoechst (blue; left) and Myogenin (red; middle) that are then merged to show a composite of the two images (right). Solid arrows point to examples of nuclear myogenin (positive) and dotted arrows point to examples of cells lacking myogenin staining (negative). Scale bar, 50 µm; (b) Bar graphs quantifying the proportion of primary myoblasts with nuclear myogenin staining after 24 h in differentiation media when exposed to tethered IgG1 (white) or tethered Jagged-1 (Jag1; gray) in the context of 12 or 42 kPa hydrogels and with (+) or without (−) treatment with DAPT or ROCKi. n = 3, P < 0.05 by two-way ANOVA.